Fig. 5.
TMF fails to induce AHR binding to its cognate response element. A, In vitro translated AHR and ARNT were incubated with vehicle (DMSO), 1 μM β-NF or increasing concentrations of TMF (0.1–20 μM) TMF and 32P-labeled DRE probe added. Samples were resolved on 6% nondenaturing retardation gels and visualized through autoradiography. Specific AHR/ARNT/DRE-retarded bands are indicated. B, in vitro translated AHR and ARNT were incubated with vehicle (DMSO), 1 μM β-NF, or 1 μM β-NF together with TMF (1 or 5 μM) and 32P-labeled DRE probe added. Samples were resolved on 6% nondenaturing retardation gels and visualized through autoradiography. Specific AHR/ARNT/DRE-retarded bands are indicated.