Fig. 1.

Baculovirus gene expression system in insect cells and silkworm larvae. a AcMNPV expression system using the AcMNPV bacmid. The target gene was incorporated into the AcMNPV bacmid. This extracted recombinant AcMNPV bacmid from E. coli was transfected into insect cells. The resulting recombinant AcMNPV was purified, amplified, and increased in titer and then was used for insect cell infection. b Conventional BmNPV expression system. The target gene and the wild-type BmNPV gene were cotransfected into B. mori cells, and the recombinant BmNPV was obtained. This recombinant BmNPV bacmid from E. coli was injected directly into silkworm larvae. After 4–6 days postinjection, the recombinant protein was harvested