Table 2.
Expression of cell/viral surface proteins and GPCRs in silkworm
| Receptor | Gene transfer | Yield | Expression level | Application | Functional assay | Function | Reference |
|---|---|---|---|---|---|---|---|
| KIR2DL1 | BmNPV Bacmid | ~0.2 mg/larva | SPR, sugar characterization | Confirmed | Sasaki et al. 2009 | ||
| IL4-Rα | BmNPV | 0.11 mg/ml hemolymph | SPR, sugar characterization | Confirmed | Honjo et al. 2008 | ||
| IL4-Rα-Fca | 0.053 mg/ml hemolymph | Gel filtration | |||||
| IL13-Rα1 | 0.55 mg/ml hemolymph | ||||||
| IL13-Rα1-Fca | 0.33 mg/ml hemolymph | ||||||
| Influenza hemagglutinin | BmNPV | 0.4–4 μg/larva | ELISA | Not confirmed | Sugiura et al. 2001 | ||
| Nociceptin receptor (Giα1)b | BmNPV bacmid | Not determined | [35S]GTPγS binding assay (EC50=9.3 ± 3.4 nM) | Kajikawa et al. 2009 | |||
| µ-Opioid receptors | Transgenic | 150–250 ng/larva | [3H]diprenorphine saturation analysis (K d=1.4~2.1 nM) | Tateno et al. 2009 |
Expression of immune cell and viral surface receptors using silkworm
SPR surface plasmon resonance analysis
aFc fusion proteins
bGα-fusion proteins