Table 1.
Single turnover ATPase activities of DnaK mutant proteins and their ATPase domain fragments
|
kcat of ATP hydrolysis (s−1)
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Basal
|
+Peptide substrate
|
+DnaJ
|
+ DnaJ/σ32
|
|||||
| Full length | ATPase domain | 100 μM | 50 nM | 0.5 μM | 2.5 μM | 10 μM | 50 nM/2 μM | |
| DnaK+ | 6.3 × 10−4 | 5 × 10−4 | 0.013 | 5.5 × 10−3 | 0.06 | >0.42 | >0.42 | 0.088 |
| YND145,147,148AAA | 2.2 × 10−3 | 6.7 × 10−4 | 0.012 | 3.2 × 10−3 | 8.8 × 10−3 | 0.01 | 9 × 10−3 | 0.011 |
| EV217,218AA | 2.7 × 10−3 | 5 × 10−4 | 0.011 | 3.7 × 10−3 | 6.8 × 10−3 | 0.02 | 0.03 | 5.8 × 10−3 |
| DnaK 1–538 | 1.0 × 10−3 | ND | ND | ND | 0.10 | >0.16 | ND | ND |
ND, not determined