Table 3.

Effects of DnaJ and GrpE on the steady–state ATPase activity of DnaK channel mutants

	Stimulation factors and kcat of ATP hydrolysis, s−1
	Basal	+DnaJ	+DnaJ/GrpE
DnaK+	1 (7.2 × 10−4)	20	240
YND145,147,148AAA	1 (1.8 × 10−3)	2	24
EV217,218AA	1 (2.5 × 10−3)	2	12

The stimulation factors are relative to the basal ATP hydrolysis rates, which are given in parentheses.