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Interdomain communication of DnaK channel mutants
|
koff of DnaK bound peptide, s−1
|
Tryptophan fluorescence, λmax [nm]
|
|||
|---|---|---|---|---|
| −ATP | +ATP | ADP | ATP | |
| DnaK+ | 1.17 × 10−3 | 2.31 (2,000-fold) | 344.6 | 341.1 |
| YND145,147,148AAA | 1.83 × 10−3 | 1.23 (700-fold) | 345.4 | 342.6 |
| EV217,218AA | 1.17 × 10−3 | 0.24 (200-fold) | 344.6 | 342.5 |
