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PEDF (50 ng/ml) strongly inhibits apoptosis in retinal spheroids. Cryosections of 10-day-old spheroids were stained with TUNEL (b, d). For calculation of the percentage of apoptotic cells, cell nuclei were stained with DAPI (a, c). The percentage of apoptotic cells was 32% in controls but only 8% in PEDF-supplemented cultures (e). Each data point represents the mean ± SD of multiple sections (n = 6). **P < 0.001. Scale bar, 100 μm
