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As photoreceptors die, resident microglia migrate into the outer nuclear and outer segment layers. Retinal sections were taken from control (a) and experimental retinas exposed to constant light for 24 h (b), 48 h (c), and 72 h (d) and immunolabeled with the microglia-specific antibody, 4C4 (arrowheads), and counterstained with 4′,6-diamidino-2-phenylindole. Scale bar = 50 μm. OSL, outer segment layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer
