Figure 6.

Molecules 1-3, but not molecule 4, promote displacement of [³H]LOS from [³H]LOS^·tCD14-His₆. [³H]LOS^··tCD14-His₆ was formed by pre-incubation of tCD14-His₆ with [³H]LOS^·_agg (0.8 nM) + LBP (4 pM), and then incubated an additional 30 min at 37° C ± 10 μM molecules 1-4, as indicated. The amount of remaining [³H]LOS^·tCD14-His₆ was assayed by co-capture of [³H]LOS To HISLINK resin as described under Experimental Procedures. Non-specific binding of [³H]LOS_agg incubated in the absence of ^·tCD14-His₆ was < 9% and subtracted from each of the experimental samples shown. Results shown represent the mean of 3 experiments each in duplicates and data are expressed as mean ± SEM. ***indicates conditions in which incubation with the indicated synthetic molecule resulted in a statistically significant (p < 0.01 (ANOVA; Dunnett’s test)) reduction in co-capture of [³H]LOS (i.e., retention of [³H]LOS.tCD14-His₆).