Table 1. Identified Secreted Proteins: WT vs. vps4Δ mutant.
Proteins were identified by searching the un-interpreted CID spectra against the NCBInr database (NCBInr_20060418) using Mascot (Matrix Science Inc, Boston MA, version 2.1.0). Cross-correlation of the Mascot results with X! Tandem and determination of protein identity probabilities was accomplished by Scaffold™ (Proteome Software Inc, Portland OR, version 2.02.03).
| Spot # | Protein Information | Gene # | Gene Name | Downregulated in Δvps4 |
ACC |
|---|---|---|---|---|---|
| 1 | similar to heat shock protease protein; Hsp5; Hsp20 region | 19.8442 | 46442969 | ||
| 2 | secreted chitinase | 19.7586 | CHT3 | X | 46441161 |
| 3 | involved in translocation of nascent polypetides across the ER membrane and in nuclear fusion during mating |
19.9564 | KAR2 | 46441414 | |
| 4 | involved in translocation of nascent polypetides across the ER membrane and in nuclear fusion during mating |
19.9564 | KAR2 | 46441414 | |
| 5 | Mp65p, potential glycosyl hydrolase [16] | 19.1779 | MP65 | X | EAK95313 |
| 6 | Mp65p, potential glycosyl hydrolase [16] | 19.1779 | MP65 | X | EAK95313 |
| 7 | Mp65p, potential glycosyl hydrolase [16] | 19.1779 | MP65 | X | EAK95313 |
| 8 | Hsp70p aka Ssa4p aka Ssa1p | 19.4980 | HSP70 | 68467506 | |
| 9 | Ssb1 in SSB subfamily of HSP70 | 19.13724 | SSB1 | 68479520 | |
| 9 | Co-chaperonin interacts with Cdc37p and Crk1p, chaperonin binding motif | 19.10702 | STI1 | 46436331 | |
| 9 | mitochondrial import protein, DNAK, HSP70 and ftsA regions | 19.9452 | SSC1 | 68485837 | |
| 10 | Ssb1p in SSB subfamily of HSP70 | 19.13724 | SSB1 | 68479520 | |
| 11 | Hsp60p, mitochondrial chaperonin, GroEL type 1 chaperonin | 19.717 | HSP60 | 68486010 | |
| 12 | Ssb1p in SSB subfamily of HSP70 | 19.13724 | SSB1 | 68479520 | |
| 12 | Hsp70p aka Ssa4p aka Ssa1p | 19.4980 | HSP70 | 68467506 | |
| 12 | mitochondrial import protein, DNAK, HSP70 and ftsA regions | 19.9452 | SSC1 | 68485837 | |
| 13 | pyruvate decarboxylase | 19.10395 | PDC11 | 68480872 | |
| 14 | pyruvate decarboxylase | 19.10395 | PDC11 | 68480872 | |
| 14 | Co-chaperonin interacts with Cdc37p and Crk1p, chaperonin binding motif | 19.10702 | STI1 | 46436331 | |
| 14 | mitochondrial import protein, DNAK, HSP70 and ftsA regions | 19.9452 | SSC1 | 68485837 | |
| 15 | dihydrolipoamide acetyl transferase (mitochondrial) | 19.6561 | LAT1 | 76573599 | |
| 16 | dihydrolipoamide acetyl transferase (mitochondrial) | 19.6561 | LAT1 | 76573599 | |
| 17 | pH regulated protein (formerly mp58p) [16] | 19.3111 | PRA1 | X | EAK96466 |
| 18 | pH regulated protein (formerly mp58p) [16] | 19.3111 | PRA1 | X | EAK96466 |
| 19 | RNA recognition motif. Predicted ORF in Assemblies 19, 20 & 21; induced in core caspofungin response; increased expression observed in an ssr1 homozygous null mutant; induced by nitric oxide in yhb1 mutant |
19.3932 | 46443783 | ||
| inorganic pyrophophatase | 19.11072 | IPP1 | 46436055 | ||
| 20 | Unidentified | X | |||
| 21 | putative mitochondrial matrix dihydrolipoamide. Putative dihydrolipoamide dehydrogenase; soluble protein in hyphae; antigenic during human oral infection and murine systemic infection; macrophage-induced protein |
19.6127 | LPD1 | 68487571 | |
| 22 | putative mitochondrial matrix dihydrolipoamide. Putative dihydrolipoamide dehydrogenase; soluble protein in hyphae; antigenic during human oral infection and murine systemic infection; macrophage-induced protein |
19.6127 | LPD1 | 68487571 | |
| 23 | Unidentified | X | |||
| 24 | involved in translocation of nascent polypeptides across the ER membrane and in nuclear fusion during mating |
19.9564 | KAR2 | 46441414 | |
| 25 | similar to heat shock protease protein; Hsp5; Hsp20 region | 19.8442 | 46442969 | ||
| 26 | similar to S. cerevisiae Sun4p (septation role) hyphal induced; caspofungin repressed; Efg1p, Cph1p regulated; predicted signal sequence, O-glycosylation, potential Kex2p substrate; 9 5′ E-boxes; 5′- UTR intron |
19.11124 | SUN41 | X | 68489582 |
| 27 | Predicted ORF in Assemblies 19, 20 and 21; regulated by Tsa1p, Tsa1Bp under H2O2 stress conditions [16] |
19.10993 | LDG8 | 46432072 | |
| 28 | putative flavodoxin WrbA, fungal specific, similar to S. cerevisiae Pst2p | 19.5285 | PST3 | 46431555 | |
| 29 | ATP synthase subunit D (normally mitochondrial). Putative subunit of the F1F0-ATPase complex; shows colony morphology-related gene regulation by Ssn6p; macrophage-downregulated protein abundance |
19.2785 | ATP7 | 68488854 | |
| 30 | glyceraldehyde-3-dehydrogenase | 19.6814 | TDH1 | 2494636 | |
| 31 | Enolase [16] | 19.395 | ENO1 | 46433227 |