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. Author manuscript; available in PMC: 2010 Jan 6.
Published in final edited form as: Cancer Prev Res (Phila). 2008 Dec;1(7):554–561. doi: 10.1158/1940-6207.CAPR-08-0040

Figure 2.

Figure 2

GSE inhibits VEGFR2 kinase activity. (A) GSE inhibited VEGFR2 kinase activity. VEGFR2 and various concentrations of GSE were incubated in kinase reaction buffer containing ATP in 96-well plates coated with a poly-Glu-Tyr substrate. Phosphorylation of the substrate was monitored with a purified phosphotyrosine specific monoclonal antibody conjugated to HRP followed by chromogenic reaction with HRP substrate. Data were represented as percentage of control that was not treated with GSE. (B) Lineweaver-Burk plot and (C) Dixon plot of the inhibition of VEGFR with GSE. Increased concentration of ATP was incubated with VEGFR2 and various concentration of GSE and monitored for their effect on kinase activity.