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. Author manuscript; available in PMC: 2010 Jan 6.
Published in final edited form as: Cancer Prev Res (Phila). 2008 Dec;1(7):554–561. doi: 10.1158/1940-6207.CAPR-08-0040

Figure 3.

Figure 3

GSE inhibit sprouts formation from chicken aorta. (A) Chick aortic rings were placed in Matrigel gels and fed with MCDB131 serum free medium containing various concentration of GSE in the presence or absence of ECGS (50μg/ml). The effect of GSE on endothelial cell sprouts formation from various aorta samples were examined on day 3. (B) Quantification of sprouts formation. Results were represented as percentage of control that was not treated with GSE. (C) Chicken aortic rings were incubated with or without GSE (10μg/ml) in presence of ECGS. The sprouts formation was examined on day 1, day 2 and day 3. (D) Inhibition of GSE on vessel sprout formation is reversed once GSE is removed from the medium. Aorta rings were first incubated with (panel II) or without GSE (10μg/ml) (panel I) for 48 hrs. The culture was then replaced with fresh medium without GSE and further incubated in the presence of ECGS for 72 hours (panel III).