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. 2009 Dec 4;10:144. doi: 10.1186/1471-2202-10-144

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Copyright ©2009 Macias et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Co-localization of BDNF (red) and synaptophysin (green) immunofluorescence in double stained cross-sections of lumbar (L3/L4) segments of the spinal cord in the intact (left column), spinal (middle column) and spinal trained (right column) rats. A. Two-color immunofluorescence photomicrographs demonstrate virtual lack of co-localization of BDNF (red) and synaptophysin (green) in the ventral quadrant of L3/L4 segment of the spinal cord in all experimental groups. Scale bar, 100 μm. B. Representative single confocal scan (1 μm thick), confirming rare co-localization of BDNF and synaptophysin (arrows) in neuropil of motor nuclei. MN- large neurons of lamina IX. Scale bar, 12.5 μm.