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. 2009 Dec 4;10:144. doi: 10.1186/1471-2202-10-144

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Copyright ©2009 Macias et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Images of the spinal cord tissue sections with delineated areas which were subject to quantitative analysis. A. Brightfield photomicrograph showing immunocytochemical labeling of BDNF on a cross section of the lumbar (L3/L4) segment of the spinal cord in the intact rat. Spinal cord laminae distinguished in L4 segment [27] are overlaid. Areas covering motor nuclei (lamina IX) delineated with dashed black lines were taken for quantitative evaluation of BDNF immunoreactivity. B. Photomicrograph showing immunofluorescence of synaptophysin around a large neuron in the ventral horn of the lumbar (L3/L4) segment of the spinal cord. Delineated areas (white lines) surrounding large neurons of lamina IX were taken for quantitative estimation of the synaptophysin immunofluorescence intensity.