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Securin with a single lysine can support efficient APC-dependent degradation. (A, C, and D) Degradation of securin and its mutants in cell extracts. 35S-labeled proteins were degraded in cell extracts prepared from Hela S3 cells synchronized in G1 phase. APC inhibitor Emi1 was added as indicated. (B) Mono-ubiquitination of securin and its mutants. 35S -labeled proteins were ubiquitinated in the presence of methylated ubiquitin. The reactions were analyzed by autoradiography.
