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Half-life of aconitase activity of S138 IRP1 mutants upon exposure to oxygen. IRP1 mutants were overexpressed and purified from yeast and subjected to in vitro cluster reconstitution anaerobically. Aconitase activity was measured at various times after diluting the reconstituted protein into air-saturated buffer. The half-life (t1/2) of aconitase activity for each IRP1 mutant is indicated in the inset.
