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(A) Structure of the porcine RI·RNase A complex (PDB entry 1dfj (9)). (B) Immunoblot of a lysate (30 μg total protein) from HeLa, K-562, and Hep-3B cells transfected with pGE-neg or pGE-pos, and probed with anti-RI or anti-actin antibodies. (C) Immunoblot of RI (5–100 ng) probed with an anti-RI antibody. (D) Bar graphs showing quantitation of the data in panels A and B. Open bars, pGE-neg; filled bars, pGE-pos. Values indicate the extent of knockdown.
