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. Author manuscript; available in PMC: 2011 Jan 12.
Published in final edited form as: Biochemistry. 2010 Jan 12;49(1):236–246. doi: 10.1021/bi901725x

FIGURE 2. Interaction of sCD-MPR constructs with a lysosomal enzyme.

FIGURE 2

SPR analysis of β-glucuronidase binding to immobilized sCD-MPR (A) and E19Q/K137M (B) constructs. sCD-MPR and E19Q/K137M were immobilized on the surface of a CM5 sensor chip to a level of 800 and 770 response units, respectively. β-glucuronidase was injected in a volume of 80 μl over the sensor surface at a rate of 40 μl/min. After 2 min, the solution containing β-glucuronidase was replaced with buffer and the complexes were allowed to dissociate for 2 min. Various concentrations of β-glucuronidase (8, 10, 20, 40, 80, 100, and 120 nM) were injected over the immobilized receptors and the resulting sensorgrams are shown.