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Liquid Chromatography Method Summary
| Column: | Phenomenex Luna 5u NH2 100A 250×4.60 mm | |||
| Elute Solvent A: | Acetonitrile with 0.1% formic acid | |||
| Elute Solvent B: | H2O with 0.1% formic acid | |||
| Data Window Start: | 15 sec | |||
| Data Window Length | 300 sec | |||
| Step: | 1 | 2 | 3 | 4 |
| Time (sec): | 30 | 120 | 60 | 120 |
| Flow Rate (mL/min): | 1.8 | 1.2 | 1.2 | 1.8 |
| Flow Type: | Step | Ramp | Ramp | Ramp |
| % Elute A | 80.0 | 30.0 | 70.0 | 80.0 |
| % Elute B | 20.0 | 70.0 | 30.0 | 20.0 |
