Figure 5.

Profound and reversible multilocularization occurs by β₃-adrenoceptor CL316,243 in the adipocytes of adult adipose tissues. The indicated adipose tissues were harvested from C57BL/6J mice that had treated with PBS (Control) or CL316,243 (~0.1 mg/kg/day) for 3 weeks (A, B), or that had discontinued the CL316,243 treatment for 1 week (Discon 1 week) or 2 week (Discon 2 week) after the CL316,243 treatment for 3 week (C, D). (A, C) Tissues were whole-mounted, co-immunostained for perilipin and collagen IV, and merged. Note that CL316,243-induced multilocular adipocytes reversibly recovered to unilocular adipocytes upon discontinuation of treatment. Scale bars, 20 µm. (B, D) Multilocular adipocytes were counted in 10 regions (~100 adipocytes/region) per adipose tissue in each mouse treated with had been treated with Control (n = 3), CL316,243 for 3 week (n = 4), Discon 1 week (n = 4) or Discon 2 weeks (n = 4), and presented as a percentage of the total counted adipocytes. Bars represent the mean ± SD. ^*P < 0.01 versus control. ^#P < 0.05 versus CL316,243 3 week.