Figure 3.

(A) Schematic representation of the different integrase expression and the donor plasmids used to in the subsequent long-term expression assays. Effect of different promoters and poly A sites on φC31 integrase mediated long-term expression in A549 (B, D) and MLE-12 (C, E) cells. Cells were transfected with 200 ng of pEGFPLucattB and equal number of molecules of the different integrase encoding plasmids. pVAX1 was used as vector control and also filler DNA to make the total amount of DNA (800 ng) equal in all the compared samples. Luciferase and total protein were measured at different time points. Long-term expression results are expressed as percentage of luciferase values two days after transfection. Each transfection was repeated twice with two independent replicates per experiment. Values represent mean ± standard deviation (n = 4). EV. Vector control.