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. 2009 Dec 31;41(12):919–934. doi: 10.3858/emm.2009.41.12.098

Figure 5.

Figure 5

Gene silencing after integration with φC31 integrase is bacterial backbone dependent in MLE12 cells. (A) Schematic representation of the plasmid DNA constructs used in this set of experiments. (B) MLE12 cells were transfected either with pEGFPLucattB ± pVAX1-Int or pCCMVI-luc-attB ± pVAX1-Int. AZA and TSA were added at 21 days post transfection. Luciferase activity was measured at different time points. Long-term expression results are expressed as percentage of luciferase values two days after transfection. (C) MLE12 cells were transfected with pEGFPLucattB ± pVAX1-Int. Increasing concentrations of AZA were added at 21 days post transfection and luciferase measurements made 48 h subsequently. (D) MLE12 cells were transfected with pEGFPLucattB ± pVAX1-Int. Increasing concentrations of TSA were added at 21 days post transfection and luciferase measurements made 48 h subsequently. Each transfection was repeated twice with two independent replicates per experiment. Values represent mean ± standard deviation (n = 4). Int: Integrase construct; EV: pVAX1 used as vector control.