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Ex vivo enzyme-linked immunospot–interferon-γ (ELISPOT–IFN-γ) responses in the three responder patients. Ex vivo ELISPOT–IFN-γ was performed as described in Materials and methods using peripheral blood mononuclear cells (PBMC) (4·105 cells/well) incubated with different peptides from E6 and E7 proteins (Table 2). The number of spots was calculated per million of PBMC.
