Figure 1.

Cross-linking analysis of PDGF β receptor in C127 cells. Extracts of PDGF-treated or untreated normal or E5-transformed C127 cells were prepared and treated with the chemical cross-linker BS³ or left untreated. Total PDGF β receptor was immunoprecipitated with αPR and detected by immunoblotting with αPR (A) or anti-phosphotyrosine antibody (B). In B, approximately 3-fold more extract was loaded in lanes 5 and 6 as in the other lanes to facilitate visualization of activated PDGF β receptor species. The position of monomeric mature (m) and precursor (p) PDGF β receptor is shown, as is the position of dimeric receptor (d).