Fig. 2.

C5a generation using sera from mice genetically deficient in complement components. Plates coated with four monoclonal antibodies (mAb) specific for bovine type II collagen were incubated with 1:10 dilutions of wild-type (WT) mouse sera or with sera deficient in various complement components: C3^−/− = sera deficient in C3; NOD = non-obese diabetic mouse, naturally deficient in C5; MBL^−/−/Df^−/− = deficient in both mannose-binding lectin (MBL) and factor D (only classical pathway intact); C1q^−/−/Df^−/− = deficient in both C1q and factor D (only lectin pathway intact); C1q^−/−/MBL^−/−- = deficient in both C1q and MBL (only altenative pathway intact). C5a generation was measured in the supernatants and expressed as optical density units. The data represent the mean ± standard error of the mean based on n = 5 for each type of mouse serum.