Table 3.

Normalized data for C3a and C5a levels using mouse and human sera.^†

	Mouse sera (%)	Human sera (%)
C3a levels
No mAb to factor B with:
Ca++ sufficient buffer (both CP and AP)	100	100
Ca++ deficient buffer (AP only)	76.6 ± 3.6*	88.9 ± 4.5*
(range)	(33–106)	(45–140)
mAb to factor B with:
Ca++ sufficient buffer (CP only)	60.5 ± 3.3**	71.0 ± 2.8**
(range)	(33–91)	(43–102)
Ca++ deficient buffer (no CP or AP)	0	5.3 ± 1.1
C5a levels
No mAb to factor B with:
Ca++ sufficient buffer (both CP and AP)	100	100
Ca++ deficient buffer (AP only)	79.1 ± 3.6***	62.8 ± 3.8***
(range)	(14–116)	(18–110)
mAb to factor B with:
Ca++ sufficient buffer (CP only)	46.6 ± 2.8****	74.3 ± 2.2****
(range)	(4–92)	(48–94)
Ca++ deficient buffer (no CP or AP)	0	0.8 ± 0.6

P-values for mouse versus human sera:

^*P = 0·04;

^**P = 0·02;

^***P = 0·003;

^****P < 0·0001.

^†The data for up to six experiments were normalized to 100% of the values obtained in the absence of monoclonal antibody (mAb) to factor using Ca⁺⁺ sufficient buffer.

The data are expressed as mean ± standard error of the mean based on n = 34 human sera for both assays, n = 45 murine sera for C5a levels and n = 24 murine sera for C3a levels. AP, alternative pathway; CO, classical pathway.