Figure 3.

3′ RACE products. 3′ RACE with the mouse-specific primer mOpn4 8F generated two fragments of 857 bp and 930 bp. The nucleotide sequence of each fragment is shown with the deduced amino acid sequence below. Exon boundaries are delineated with vertical blue lines, potential polyadenylation signals are underlined and potential protein kinase C sites are boxed. A, The 857 bp fragment consists of 425 bp of coding sequence (104 bp of exon 8 and 321 bp of exon 9) and 432 bp of 3′ UTR contiguous to exon 9 and corresponds to Opn4L. B, The 930 bp fragment is composed of 260 bp of coding sequence split across 3 exons (104 bp of exon 8, 117 bp of exon 9 and 39 bp of exon 10). The remaining 670 bp of 3′ UTR is contiguous to the newly identified exon 10. This product corresponds to Opn4S.