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Opn4 localization in RGC-5 cells. RGC-5 cells were transfected with Opn4L (A) and Opn4S (B) and fixed after 24 h. Melanopsin expression was detected using a rabbit anti-Opn4 antibody targeted to the N terminus of the protein (PA8331), and immunofluorescence signal was observed mainly on the plasma membrane (arrows). Scale bar, 10 μm.
