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. Author manuscript; available in PMC: 2010 Apr 17.
Published in final edited form as: Cell. 2009 Apr 17;137(2):259–272. doi: 10.1016/j.cell.2009.02.045

Fig 1. Set1 mediates a reduction in histone acetylation of 5′ regions.

Fig 1

Cross-linked chromatin from wild-type, set1Δ, or set2Δ strains (indicated above each panel) was precipitated with anti-H3, anti-acetyl H4, or anti-acetyl H3 (indicated below panels). PCR analysis of the precipitated DNA was carried out on the YEF3 (A), PMA1 (B), and PYK1 and ADH1 (C) genes (numbered primer locations shown schematically at the top, PCR products below). TEL is from a non-transcribed region near the telomere of chromosome VI.

D. The signals for acetyl H4 or acetyl H3 were quantitated and normalized to the total H3 signal and the ratios were graphed (X axis shows primer number and Y axis shows ratio). Error bars show the standard deviation from at least three independent experiments.

E. Quantitation for the telomere primer pair was carried out as in D.