Skip to main content
. Author manuscript; available in PMC: 2010 Apr 17.
Published in final edited form as: Cell. 2009 Apr 17;137(2):259–272. doi: 10.1016/j.cell.2009.02.045

Fig 2. H3K4me2 mediates reduced histone acetylation of 5′ regions.

Fig 2

A. ChIP from wild-type, rad6Δ, or bre1Δ strains using anti-H3, anti-H3K4me3, anti-H3K4me2, or anti-H3K4me1 (left panels) and anti-H3, anti-acetyl H4, or anti-acetyl H3 (right panels) were carried out on YEF3. Primer locations are shown schematically at top. Similar results were seen at all other genes tested.

B. The results from part A for acetyl H4 and acetyl H3 were normalized to the total H3 signal and the ratios were graphed. Error bars show the standard deviation from multiple experiments.

C. Vector or plasmids expressing wild-type Set1 or a mutant Set1 lacking the RRM domain (ΔRRM) were transformed into a set1Δ strain and ChIP for histone modifications was carried out as in parts A and B.

D. Results from part C were quantitated and graphed as in part B.