Figure 4.

The habenula develops from Dbx1-expressing precursors in the presumptive dorsal thalamus. A, Schematic of the brain at E12.5 showing approximate plane of section for subsequent views. B, C, Immunofluorescence for Pax3, Dbx1, and Olig3 reveals nearly discrete domains in the neuroepithelium of the presumptive thalamus. D–F, Immunofluorescence for Brn3a with each of the progenitor domain markers reveals an association of the habenula with Dbx1-expressing precursors. Scale bars: B, C, 100 μm; D–F, 50 μm. thal, Thalamus; hab, habenula.