Figure 6.

Molecular phenotype of the habenula in Brn3a null mice. Expression of potential Brn3a target genes identified by microarray were examined in Pou4f1^+/+ and Pou4f1^−/− embryos at E16.5 using ISH. In general, the magnitude of the changes in expression detected by ISH appeared to be greater than the changes observed on the array, and transcripts with more than fourfold decreased expression on the microarray were generally undetectable by ISH in Pou4f1^−/− embryos. This may be in part because the use of heterozygous controls in the microarray study somewhat reduced the magnitude of the changes in expression measured by that method. DCC expression was verified but restricted to cells immediately adjacent to the ventricle (arrowheads). Nrp2 expression appeared unchanged, but Nrp2-expressing cells were displaced toward the midline, as observed for other markers (arrows). Expanded gene names appear in Table 2. Hip, Hippocampus; CP, choroid plexus. Scale bar, 100 μm.