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. 2009 Oct 7;29(40):12467–12476. doi: 10.1523/JNEUROSCI.3180-09.2009

Figure 5.

Figure 5.

CCL8 downregulated EGR1 in differentiated SK-N-MC cells. A, Culture supernatant from SIV-infected macrophages inhibited both constitutive and NGF-induced (30 min treatment) EGR1 expression in 4 d differentiated SK-N-MC cells after 2 d pretreatment at 1:50 supernatant dilution. Normalization was done using housekeeping genes 18S and GAPDH; the figure shows expression in terms of relative units. B, In cells cultured at the same time and under the same conditions as for A, constitutive and NGF-induced (2 h treatment) EGR1 protein was also reduced by 2 d pretreatment with 1:50 dilution of supernatant from infected macrophages. C, Mean EGR1 expression (normalized to 18S and GAPDH; relative units) ± SEM of five experiments in which 5 d differentiated SK-N-MC cells were treated for 2.5–3 h with one of the indicated chemokines at 100 ng/ml is presented. Inhibition of EGR1 by CCL8 was significant at p < 0.01 as assessed by paired sample one-way ANOVA (Prism: GraphPad) performed on log-transformed data followed by Dunnett's posttest.