Figure 8.

Polyribosome association of VEGF mRNA in response to TPA and RA + TPA. HESC were incubated with TPA (50 nm), TPA (50 nm) + RA (1 μm), or solvent control for 6 h. The cytoplasmic extracts were subjected to linear sucrose gradient fractionation in parallel and each treatment yielded ten 1-ml fractions. Total RNA was isolated from each fraction and subjected to qRT-PCR as described in Materials and Methods. The percentage of total VEGF mRNA in each gradient fraction was calculated and graphically displayed. *, Significant increase in percent of total VEGF mRNA in the indicated fractions compared with controls.