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. 2009 Nov 3;24(1):91–103. doi: 10.1210/me.2009-0279

Figure 2.

Figure 2

T220D Pit-1 homeodomain fails to bind to dimeric FPI (Prl-1P) probe. In each 20-μl reaction, 300 ng of WT and mutant GST-Pit-1 homeodomain (AA 199-291) fusion proteins were incubated with 28,000 cpm of the FPI probe in binding buffer and 1 ng polydeoxyinosinic deoxycytidylic acid. After a 30-min incubation at room temperature, 2 μl of loading buffer was added to each sample and ran on preelectrophoresed 6% nondenaturing polyacrylamide gel in 0.25× TBE buffer at 190 V. After electrophoresis, the gel was dried and visualized by autoradiography. Specific GST-Pit-1 homeodomain DNA complexes are labeled A and B/C.