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. 2009 Nov 6;24(1):178–192. doi: 10.1210/me.2009-0167

Figure 1.

Figure 1

Igfbp5 mRNA and protein is up-regulated in the myrAkt1 TG islets, and expression is absent in Igfbp5−/− islets. A, Islets from 4- to 6-wk-old mice were isolated and total RNA was extracted and assessed by quantitative PCR for Igfbp5. Igfbp5 mRNA was corrected for Gapdh expression (n = 3 per genotype). Error bars represent sd. *, P < 0.01. B, Islets were isolated from 12- to 14-wk-old mice, and protein lysates were prepared from a total of 30 islets per genotype and processed for immunoblotting as described in Materials and Methods. WT, Wild-type; P-Akt, phospho-Akt.