Figure 2.

Igfbp5 expression is increased in MIN6 cells by stable expression of myrAkt1. MIN6-myrAkt1 cells were plated into six-well plates and harvested at approximately 80% confluency after an overnight culture in DMEM containing 3 mm glucose. A, Cell extracts were processed for immunoblotting as described in Materials and Methods. B, Total RNA was extracted, and Igfbp5 expression was assessed by quantitative PCR. Igfbp5 mRNA level was corrected for TBP expression. The graph reflects a representative experiment from a total of three separate experiments performed in triplicate. Error bars represent sd. *, P < 0.01. P-GSK, Phospho-GSK.