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MyrAkt1 induces Igfbp5 mRNA expression in primary MEFs. Primary MEFs were infected with either ad-GFP or myrAkt1 and then subcultured for 48 h before harvesting. A, Cells were lysed and processed for immunoblotting as described in Materials and Methods. B, Igfbp5 expression was assessed by quantitative PCR. Igfbp5 mRNA levels were normalized to TBP expression. The graph represents the average of four separate experiments. The error bars indicate sd. *, P < 0.001. P-Akt, Phospho-Akt.
