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. 2009 Nov 3;24(1):76–90. doi: 10.1210/me.2009-0218

Figure 2.

Figure 2

Effect of BRCA1 knockdown on activity of acetylation-site mutant ER-α proteins. A, DU-145 cells were transfected with the indicated ER-α expression vector and the ERE-TK-Luc reporter, exposed to the indicated concentration of E2 for 24 h, and harvested for luciferase assays. B–D, DU-145 cells were treated with BRCA1 siRNA (50 nm), control (CON) siRNA (50 nm), or no siRNA (vehicle only) for 48 h; transfected with the indicated ER-α vector and the ERE-TK-Luc reporter overnight; exposed to 0 E2 (B), 1 nm E2 (C), or 10 nm of E2 (D) for 24 h; and harvested for luciferase assays. In all panels, luciferase activity is expressed relative to control conditions (0 E2, wt-ER-α) as means ± sem of quadruplicate wells.