Figure 7.

Effect of acetylation-site mutations on BRCA1 regulation of ER-α acetylation. DU-145 cells were pretreated with the indicated siRNA (50 nm) for 48 h, transfected with wt-ER-α or mutant ER-α, and harvested for immunoprecipitation (IP)-Western blotting to determine the levels of acetylated ER-α and total ER-α. Alternatively, the cells were cotransfected overnight with wtBRCA1 or empty pcDNA3 vector and with wt-ER-α or mutant ER-α and then subjected to immunoprecipitation-Western blotting as above. The ER-α mutants tested were K302/303A and K303R (A) and K266/268R and K266/268Q (B and C). CON, Control.