Table 1.
Microsatellite sequences of primer pairs developed for Oryza glumaepatula with the chromosome where the loci was identified (C), allele size range (pb), number of alleles per locus (A), observed heterozygosity (Ho) and annealing temperatures (Ta)
| SSR locus | Primers sequences* | C | pb | A | Ho | Ta (°C) |
|---|---|---|---|---|---|---|
| OG-22 | (F) GCCATCCATTCTTACCAG | 12 | 175–185 (SO) | 3 | 0·000 | 56 |
| (R) CACAGGTGTGGTGCTCA | 190–225 (PG) | 7 | 0·067 | |||
| 180–200 (XI) | 5 | 0·242 | ||||
| OG-26 | (F) CATGGTGCCGATTACGGT | 10 | 95–100 (SO) | 2 | 0·012 | 60 |
| (R) CATCTCCATCGCGGTCAT | 95–110 (PG) | 4 | 0·103 | |||
| 95–110 (XI) | 4 | 0·050 | ||||
| OG-27 | (F) TCGGACGTGGCATATGA | 9 | 140–165 (SO) | 4 | 0·010 | 54 |
| (R) CTGTTCCGAGCGAGAGT | 140–210 (PG) | 6 | 0·117 | |||
| – (XI) | – | – | ||||
| 0G-29 | (F) GACCAGTTCACCATGCAG | 1 | 100–105 (SO) | 2 | 0·000 | 60 |
| (R) GAGTGAGGCAGCAAGACA | 90–130 (PG) | 7 | 0·021 | |||
| 85–105 (XI) | 4 | 0·051 | ||||
| 0G-36 | (F) AACGTTCATCGGTTCTGG | 4 | 170–195 (SO) | 5 | 0·000 | 56 |
| (R) TGCTTGCCAGGTTATTCC | 180–195 (PG) | 4 | 0·098 | |||
| 160–170 (XI) | 3 | 0·280 | ||||
| OG-39 | (F) GCGTACTAGGCCATGATA | 3 | 250–270 (SO) | 2 | 0·000 | 56 |
| (R) TCCACGTAAGAACACTCG | 270–285 (PG) | 4 | 0·150 | |||
| 250–265 (XI) | 2 | 0·000 | ||||
| OG-42 | (F) TGCAGGCTCTGAGCTAC | 5 | 420–430 (SO) | 2 | 0·000 | 56 |
| (R) AGAACAGATCTTGCCGTC | 425–445 (PG) | 4 | 0·010 | |||
| 420 (XI) | 1 | 0·000 | ||||
| OG-63 | (F) CAGGGGACAAGCACATA | 2 | 115–145 (SO) | 4 | 0·000 | 56 |
| (R) TAGACGATGTCGAGAAGG | 100–145 (PG) | 8 | 0·202 | |||
| 130–135 (XI) | 2 | 0·041 |
*Sequences of primer pairs developed by Brondani et al. (2001).