Figure 1.

Accessibility of λ O protein in the O-some structure to ClpP/ClpX-dependent proteolysis. Increasing amounts of oriλ-containing DNA (○) or oriφ82-containig DNA (•) were added to the standard protease assay reaction mixture containing ³H-labeled λ O. After a 3-min preincubation at 30°C, ClpX and ClpP proteins were added. Incubation proceeded for an additional 5 min at 30°C, the reaction was stopped, and the amount of ³H-labeled λ O hydrolyzed was estimated as described in Materials and Methods.