Figure 5.

Both DnaB and λ P are required for the stabilization of λ O when present in the preprimosomal complex. Increasing amounts of DnaB helicase were added to the ³H-labeled λ O and oriλ DNA in the presence (•) or absence (○) of λ P. After a 3-min preincubation at 30°C, ClpP/ClpX protease, RNA polymerase, and rNTPs were added. Following a 5-min incubation at 30°C, the reactions were stopped, and the amount of λ O hydrolyzed was estimated as described in Materials and Methods.