Figure 6.

Expression of Sertoli cell differentiation markers during long-term grafting.

mRNA isolated from xenografts after 2–12 weeks was analyzed by RT–PCR (n = 3). In control experiments, PCR reactions were performed on RT reactions without reverse transcriptase (−RT) and in the absence of cDNA (no DNA). Average expression levels were normalized to Ppia, and the mean (±SEM) fold change expressed relative to 2 weeks (scaled to 1). M = 100 bp DNA ladder. Expression of Wt1 was detected throughout all time points. Expression of Krt18, Gata4, Cyp19a1 Trf, Fshr and Shbg were examined. Different lower case letters indicate statistically significant changes of expression (P < 0.05).