Table II.

Oligonucleotide primer sequences for the amplification of Sertoli cell maturation markers

Gene	Sequence	TA (°C)	Region amplified	Product size (bp)
Ppia	Forward 5′-ATGGTCAACCCCACCGTGT-3′	60	43–143	100
	Reverse 5′-TCTGCTGTCTTTGGAACTTTGTCT-3′
Trf	Forward 5′-ATCTGGGAGATCCTCAAAGTGGCTC-3′	59	874–1277	403
	Reverse 5′-GGCACTAGTCCACACTGGCCTGCTA-3′
Shbg	Forward 5′-GACGGACCCTGAGACACATT-3′	57.3	107–674	590
	Reverse 5′-GAACAGTCCAGGTTGCAGGT-3′
Wt1	Forward 5′-GCCACCCCACTCCTTCATCAAAC-3′	61.8	210–788	601
	Reverse 5′-GTTGCTCTGCCCTTCTGTCCATT-3′
Fshr	Forward 5′-TCTGTCCTGTAAATCTGGGCTTGC-3′	55	1709–2037	328
	Reverse 5′-TGCTATACCCACATCTACCTC-3′
Gata4	Forward 5′-CTGTCATCTCACTATGGGCAC-3′	56.1	1669–1905	257
	Reverse 5′-CCAAGTCCGAGCAGGAATTTG-3′
Amh	Forward 5′-CCTTTCTGTTTGGCTCTGATTCC-3′	61.2	683–1024	341
	Reverse 5′-GCGGGTGACAGCAGCAGTAATAG-3′
Krt18	Forward 5′-TCCACCACCTTCTCCACCAACTA-3′	60.8	59–308	272
	Reverse 5′-TTCTCGGTTTCCAGGTTCTTCAC-3′
Cyp19a1	Forward 5′-TGCACAGGCTCGAGTATTTCC-3′	56	1411–1714	304
	Reverse 5′-ATTTCCACAATGGGGCTGTCC-3′