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. Author manuscript; available in PMC: 2010 Aug 21.
Published in final edited form as: Immunity. 2009 Aug 21;31(2):232–244. doi: 10.1016/j.immuni.2009.06.022

Figure 2. Irgm3 does not control phagocytosis but does delay phagosomal maturation.

Figure 2

(A) Phagocytosis assay. WT or Irgm3−/− mDCs were incubated for 10 minutes with various amounts of latex beads labeled with Alexa-488 and analyzed by FACS in the presence of trypan blue to quench the fluorescence of extracellular beads. In the right panel, cells were pretreated with CytochalasinD to prevent phagocytosis.

(B, C) Phagosomal maturation assay. WT or Irgm3−/− mDCs were fed with OVA covalently linked to 3µm latex beads for 10mn, washed to remove free beads and left in culture for various time lapses. After hypoosmotic lysis and fixation, phagosomes were analyzed by flow cytometry after staining with anti-LAMP2 antibodies (B) or anti-OVA antibodies (C). A representative experiment is shown.