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. Author manuscript; available in PMC: 2010 Apr 16.
Published in final edited form as: Cell Cycle. 2009 Apr 16;8(8):1185–1195. doi: 10.4161/cc.8.8.8202

FIGURE 4. Dlx3 reduces p63 protein half-life and induces proteasome-mediated p63 degradation.

FIGURE 4

a) Extracts from Saos2 cells transfected with the indicated plasmids were treated or not with 10 μM MG132 (Z-Ieu-leu-leu-CHO). 48 hours after transfection cell extracts were prepared and subjected to Western blot analysis. p63 and Dlx3 were revealed with anti-p63 and anti-Flag antibodies, respectively. b) Saos2 cells transfected with ΔNp63α or ΔNp63αS383AT397A alone or with 1 μ.g of Dlx3-Flag vector were treated with cyclohexamide (CHX) for the indicated times. Equal amounts of total proteins were subjected to Western blot analysis for p63 and Dlx3. The plot represents the quantification of the p63 half life by densitometry scanning expressed as percentage of the remaining protein. Each profile represents the mean of three independent experiments. Standard Deviations are also shown.