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. Author manuscript; available in PMC: 2010 Apr 16.
Published in final edited form as: Cell Cycle. 2009 Apr 16;8(8):1185–1195. doi: 10.4161/cc.8.8.8202

FIGURE 6. Raf kinase activity is involved in Dlx3-induced p63 degradation.

FIGURE 6

a) Saos2 cells transfected with 0.2 μg of ΔNp63α plasmid alone (lane 1 and 2) or with 1.5 μg of Dlx3-Flag plasmid (lanes from 3 to 6) were treated with 10 μM GW5074 as indicated. At 24 hours after transfection cell lysates were blotted with antibodies to p63, Flag and actin. b) Raf1 null Mouse Embryo Fibroblasts (MEFs) were transfected with ΔNp63α plasmid (0.2 μg) alone or with the indicated amounts of Dlx3-Flag and RafBxB plasmids. At 24 hrs after transfection cell Iysates were blotted with antibodies to p63, Flag, Raf1 and actin. c) Dlx3-Flag plasmid (0.5 ug) was transfected in Saos2 cells alone or with the indicated amounts of RafBxB expression vector. 24 hours after transfection cell lysates were blotted with antibodies to Flag. Antibodies against total Raf1 were used to check for the level of RafBxB while antibodies to actin were used as loading control.