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. Author manuscript; available in PMC: 2010 Apr 16.
Published in final edited form as: Cell Cycle. 2009 Apr 16;8(8):1185–1195. doi: 10.4161/cc.8.8.8202

FIGURE 7. Raf1 cooperates with Dlx3 to induce p63 protein degradation.

FIGURE 7

a) ΔNp63α plasmid was transiently transfected in Saos2 cells alone (lane 1), or with the indicated amounts of Dlx3-Flag and RafBXB expression vectors. 24 hours after transfection cell Iysates were blotted with antibodies to p63, Flag and actin. Antibodies against Raft were used to check for the level of RafBXB. b) ΔNp63αS383AT397A plasmid was transiently transfected in Saos2 cells alone or with the indicated amounts of Dlx3 and RafBxB expression vectors. 24 hours after transfection cell lysates were blotted with antibodies to p63, Flag and actin. Antibodies against Raf1 were used to check for the level of RafBXB.