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. Author manuscript; available in PMC: 2010 Apr 16.
Published in final edited form as: Cell Cycle. 2009 Apr 16;8(8):1185–1195. doi: 10.4161/cc.8.8.8202

FIGURE 8. Dlx3 induces Ran phosphorylation.

FIGURE 8

a) HaCaT cells were mock transfected or transfected with 1.0 or 1.5 μg of Dlx3 expression vector. Total RNA was prepared from untransfected, mock or Dlx3 transfected HaCaT cells at 48 hours post-transfection. Real Time PCR was performed with Raf1 and HPRT specific oligonucleotides as described in Material and Methods. The experiment was performed twice and samples were done in triplicate. HPRT was used for normalization. The results were expressed with the value relative to HPRT (set at 1) for each mRNA sample. Standard deviations are also shown. b) Saos2 cells were transiently transfected with the indicated amounts of Dlx3 plasmid. 24 hours after transfection cell lysates were blotted with antibodies against actin, total and phosphorylated Raf1. Dlx3 was detected with antibodies to Flag. c)HaCaT cells were mock transfected or transfected with 1.0 or 1.5 ug of Dlx3 expression vector. Equal amounts of total protein (40 μg) were blotted with antibodies against p63, Flag, total (α-tRaf1) or phosphorylated Raf1 (α-pSer338Raf1). Loading equivalence was assessed with antibodies against actin.